Biolegend, 405420, Alexa Fluor® 555 Goat anti-rat IgG (minimal x-reactivity) Antibody
100microg
Catalog Number: 405420
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| Verified Reactivity | Rat |
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| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICC - Quality tested IHC-F - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser | Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
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For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes | This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies. |
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| Application References(PubMed link indicates BioLegend citation) | Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P) |
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| Product Citations | Wang JX, et al. 2023. JCI Insight. 8:. PubMed Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed Hsiao HW, et al. 2023. Biomolecules. 13: . PubMed Challa DK, et al. 2019. MAbs. 11:848. PubMed Luo L, et al. 2021. J Neuroinflammation. 18:27. PubMed Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed |
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| RRID | AB_2563180 (BioLegend Cat. No. 405420) |
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| Gene ID | 295279 |
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| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4054 |
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| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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