Biolegend, 405428, Biotin Goat anti-rat IgG (minimal x-reactivity) Antibody
100microg
Catalog Number: 405428
| Verified Reactivity | Rat |
|---|---|
| Antibody Type | Polyclonal |
| Host Species | Goat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | FC - Quality testedELISA, WB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.6 ug per million cells in 100 ul volume. In addition, it has been tested by ELISA (≤ 2 ug/ml) to assure the specificity and reactivity. It is recommended that the reagent be titrated for optimal performance for each application. |
| Application Notes | This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies. |
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| Application References(PubMed link indicates BioLegend citation) |
|---|
| Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P) |
| RRID | AB_2687099 (BioLegend Cat. No. 405428) |
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| Gene ID | 295279 |
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| UniProt | View information about IgG on UniProt.org |
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| Clone | Poly4054 |
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| Regulatory Status | RUO |
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| Isotype | Goat Polyclonal IgG |
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