Biolegend, 405729, Alexa Fluor® 700 anti-mouse IgD Antibody
25microg
Catalog Number: 405729
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested SB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11. |
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| Additional Product Notes | Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH). |
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| Application References(PubMed link indicates BioLegend citation) | Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Pani F, et al. 2021. Endocrinology. 162:. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Vijayan K, et al. 2021. Cell Reports. 36(5):109489. PubMed Yen WF et al. 2019. Cell reports. 27(5):1472-1486 . PubMed Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed Wilder BK, et al. 2022. NPJ Vaccines. 7:58. PubMed Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed Carbonetti S et al. 2017. Journal of immunological methods. 448:66-73 . PubMed Song W, et al. 2022. Immunity. 55:290. PubMed |
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| RRID | AB_2563340 (BioLegend Cat. No. 405729) AB_2563341 (BioLegend Cat. No. 405730) |
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| Structure | Ig family |
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| Distribution | B cells |
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| Function | B cell differentiation |
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| Cell Type | B cells |
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| Biology Area | Immunology |
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| Gene ID | 380797 |
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| UniProt | View information about IgD on UniProt.org |
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| Clone | 11-26c.2a |
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| Regulatory Status | RUO |
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| Other Names | Immunoglobulin D |
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| Isotype | Rat IgG2a, κ |
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