Biolegend, 405740, Alexa Fluor® 594 anti-mouse IgD Antibody
100microg
Catalog Number: 405740
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | IHC-F - Quality tested 3D IHC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes |
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| The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11. |
| Application References(PubMed link indicates BioLegend citation) | Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed |
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| Product Citations | Whyte CE, et al. 2022. J Exp Med. 219:. PubMed Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed Radice E, et al. 2020. Cell Reports. 32(5):107951. PubMed |
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| RRID | AB_2565572 (BioLegend Cat. No. 405740) |
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| Structure | Ig family |
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| Distribution | B cells |
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| Function | B cell differentiation |
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| Cell Type | B cells |
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| Biology Area | Immunology |
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| Gene ID | 380797 |
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| UniProt | View information about IgD on UniProt.org |
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| Clone | 11-26c.2a |
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| Regulatory Status | RUO |
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| Other Names | Immunoglobulin D |
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| Isotype | Rat IgG2a, κ |
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