Biolegend, 420504, Cell-Vive™ CD Low DMSO Cryopreservation, GMP
50ml
Catalog Number: 420504
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| Formulation | Chemically-defined formulation |
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| Endotoxin Level | < 1EU/mL |
| Storage & Handling | Store at 2°C to 8°C. |
| Application | Cell Culture |
| Application Notes | For cryopreservation, a range between 5-50 million cells per 1 mL of Cell-Vive™ CD Low DMSO Cryopreservation, GMP is recommended. The appearance of the product in liquid form is clear with a slight yellow color. Cryopreservation protocol Prepare an immune cells suspension following an appropriate protocol. Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully decant or aspirate supernatant Resuspend pelleted cells with Cell-Vive™ CD Low DMSO Cryopreservation, GMP and dispense the cell suspension into a cryovial. Immediately transfer the cryovial into a cell freezing container and store it at -70°C After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage. Thawing cells protocol Thaw cryovials containing cells by putting them in a 37°C water bath with very gently swirling. Thaw until only a small ice fragment is present. In a biosafety hood, add 1 mL of preferred cell culture media such as IMDM into the vial and then transfer content into a 15 mL conical tube containing 8mL of cell culture media at room temperature. Gently mix Centrifuge cells at 300 x g for 5 minutes. Carefully decant or aspirate supernatant Resuspend the cells at the desired density with cell culture media. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Gene ID | NA |
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| Other Names | Chemically-defined Low DMSO Cryopreservation Solution |
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