Biolegend, 420514, Cell-Vive™ CD RBC Lysis Buffer (10X), GMP
200ml
Catalog Number: 420514
Send an RFQ.
Category
Loading…
| Formulation | Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water |
|---|---|
| Endotoxin Level | < 1 EU/mL |
| Preparation | Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. Warm the 1X solution to room temperature before use. |
| Storage & Handling | Store between 2°C-8°C |
| Recommended Usage | Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. 2 ml of 1X Cell-Vive™ CD RBC Lysis Buffer, GMP is enough to lyse up to 100 μl of whole blood. |
| Application Notes | Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is recommended for the lysis of human red blood cells. This buffer has a chemically-defined formulation without animal-derived components, it contains low levels of endotoxin (< 1EU/mL), and it is tested negative for mycoplasma and microbial growths. Use Cell-Vive™ CD RBC Lysis Buffer (10X), GMP for highly sensitive assays. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X formulation that contains no preservatives, handle under aseptic conditions as needed. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Additional Product Notes |
|---|
| Human Red Blood Cells lysis protocol Dilute the Cell-Vive™ CD RBC Lysis Buffer (10X), GMP to 1X working concentration with deionized water. Warm the 1X solution to room temperature before use. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 μl of human whole blood. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature for 10-15 minutes. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing the pellet. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell-Vive™ CD Cell Staining Buffer, GMP Cat. No. 420508), and wash 1X. Resuspend and proceed with further procedures. |
| Disclaimer | BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
|---|
| Gene ID | NA |
|---|
| Other Names | Red blood cell lysis buffer, lysis buffer, CD buffer, RBC Buffer |
|---|
