Biolegend, 420518, Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP
50ml
Catalog Number: 420518
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| Formulation | Chemically-defined. Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is a solution ready to use. |
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| Endotoxin Level | < 1EU/mL |
| Preparation | Chemically-Defined, no preservatives. |
| Storage & Handling | 4°C (2-8 °C) |
| Application | Cell Culture |
| Recommended Usage | Freeze 0.5-5 million cells per 1mL of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP |
| Application Notes | For cryopreservation, a range between 0.5-5 million cells per 1 mL of Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP is recommended. The liquid product is clear in appearance. Cryopreservation protocol: Prepare a cell suspension following an appropriate protocol. Centrifuge cells at 300 x g for 5 minutes to obtain a pellet. Carefully aspirate supernatant Resuspend pelleted cells with Cell-Vive™ CD Blood 55-5 Cryopreservation Solution, GMP and dispense the cell suspension into a cryovial. Immediately transfer the cryovial into a cell freezing container and store it at -80°C. After 24-36 hours, transfer cryovials into a liquid nitrogen tank for long-term storage. Thawing protocol Thaw cryovials containing cells by gently swirling them in a 37°C water bath. Thaw until only a small ice fragment is present. In a biosafety hood, add the thawed cryopreservation solution into 8 mL of preferred cell culture media in a 15 mL conical tube. Wash the vial with 1 mL of cell culture media and add to the conical tube containing cells. Gently mix. Centrifuge and remove the supernatant. Resuspend the cells at the desired density with cell culture media. |
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| Disclaimer | BioLegend Cell-Vive™ GMP Cell Culture products are for research use only. Suitable for ex vivo cell processing use. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Gene ID | NA |
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| Other Names | Cryo Solution, cropreservation media |
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