Biolegend, 421906, Autophagy Detection Probe Blue
1VIAL
Catalog Number: 421906
Send an RFQ.
Category
Pack Size1 vial
Loading…
| Verified Reactivity | Human |
|---|---|
| Reported Reactivity | Mouse |
| Formulation | 1 vial of Autophagy Detection Probe (50 µL) |
| Preparation | Please refer to detailed protocol in application notes. |
| Storage & Handling | -20°C |
| Application | Live cell imaging - Quality tested |
| Recommended Usage | Please refer to detailed protocol in application notes. |
| Application Notes | This probe employs a specific autophagosome marker for detection of autophagy in live cells (adherent or suspension). Using the recommended volume of 100 μL per well in a 96-well plate, this product supplies enough reagents for 200 tests. Component: 50 μL of 100X Autophagy Probe (-20°C, protected from light, avoid freeze/thaw cycles) Required Materials Not Included: Stain Buffer: Phosphate buffer saline, pH7.2 (PBS), phenol-free media, Hanks Balanced Salt solution (HBSS), or Cell Staining Buffer (Cat. No. 420201) Imaging Guidelines: Ex/Em = 330/520 nm Fluorescence microscope filter set: DAPI Assay Protocol: Thaw the probe at room temperature before starting the experiment. Treat your cells with test compounds or conditions (e.g. starvation, Rapamycin, Chloroquine, Autophinib). Prepare the autophagy staining solution by diluting the autophagy probe 1:500 in Cell Staining Buffer (Cat. No. 420201), PBS, phenol-free media, or HBSS and mix well. Note: We recommend 100 μL per well in a 96-well plate. Remove media from cells and add the desired volume of autophagy staining solution. If cells are in suspension, spin down and resuspend. Optional: Include live-cell nuclear counterstain such as DRAQ5™ (Cat. No. 424101). Incubate cells at 37°C for 15-60 minutes. Remove autophagy staining solution. If cells are in suspension, spin down and remove solution. Gently wash cells twice with Cell Staining Buffer (Cat. No. 420201), PBS, phenol-free media, or HBSS. Immediately proceed to imaging. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Distribution | Autophagosomes |
|---|
| Biology Area | Cell Biology, Cell Structure, Protein Trafficking and Clearance |
|---|
| Molecular Family | Autophagosome Markers |
|---|
| Antigen References | Aman Y, et al. 2021. Nat Aging. 1:634–50. Dikic I, et al. 2018. Nat Rev Mol Cell Biol. 19:349–64. Khandia R, et al. 2019. Cells. 8:674. |
|---|
| Gene ID | NA |
|---|
| Regulatory Status | RUO |
|---|
