Biolegend, 421944, ATP Red
1mg
Catalog Number: 421944
Send an RFQ.
Category
Pack Size1 vial
Loading…
| Verified Reactivity | Human, Mouse |
|---|---|
| Formulation | 1 vial of lyophilized ATP Red, 1 mg |
| Preparation | Bring vial to room temperature. To make a 10 mM stock solution, add 178 µL of DMSO to the vial of 1 mg ATP Red and mix thoroughly until completely dissolved. Note: Store unused ATP Red stock solution in a tightly sealed vial in the dark at room temperature. Stock solution is stable for at least 12 months. |
| Storage & Handling | 2 - 8°C |
| Application | FC - Quality tested Live cell imaging - Verified |
| Recommended Usage | Please refer to the detailed protocol in the application notes section. |
| Application Notes | Components: 1 vial of 1 mg lyophilized ATP Red, Molar Mass: 561.48 g/mol Storage: Upon receipt, store lyophilized probe at 2 - 8°C and protected from light. Upon reconstitution, store unused ATP Red stock solution in a tightly sealed vial in the dark at room temperature. Stock solution is stable for at least 12 months. Imaging Guidelines: Ex/Em = 590/620 nm Fluorescence microscope filter set: Cyanine3/TRITC Flow Guidelines: Analysis in PE or similar channel (585/42 or 575/25 or 570/40 filter set) Protocol: Grow cells in a tissue culture vessel with a coverslip bottom in desired media or prepare cell suspension containing 0.25 - 2.5 x106 cells in 100 - 500 µL of desired media or Cell Staining Buffer (Cat. No. 420201). Optional: Fix cells with Fixation Buffer (Cat. No. 420801), if desired. Add ATP Red at a final concentration of 5 - 10 µM along with desired antibodies. Incubate cells at room temperature or 37°C for 15 minutes (or up to 30 minutes for live cell imaging). Optional: Wash cells with PBS or equivalent buffer. If only staining with ATP Red, this washing step can be skipped. Proceed directly to analysis. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Distribution | Mitochondria |
|---|
| Biology Area | Adaptive Immunity, Cell Biology, Cell Proliferation and Viability, Immunology, Innate Immunity, Mitochondrial Function |
|---|
| Molecular Family | Mitochondrial Markers |
|---|
| Antigen References | Wang L, et al. 2016. Angew Chem Int Ed Engl. 55:1773-6. |
|---|
| Gene ID | NA |
|---|
| Regulatory Status | RUO |
|---|
