Biolegend, 422501, Cyto-Last™ Buffer
100ml
Catalog Number: 422501
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| Storage & Handling | Store Cyto-Last™ Buffer between 2°C and 8°C. |
|---|---|
| Application | ICFC - Quality tested |
| Application Notes | Staining Procedure: 1. Prepare target cells of interest and perform surface staining as described in BioLegend's Cell Surface Immunofluorescence Staining Protocol. (Note: staining with tandem-dye-conjugated antibodies (e.g., PE/Cyanine7, APC/Cyanine7, etc.) is not recommended as color compensation shifts may occur with long-term storage.) 2. Fix the cells with 0.5 mL/tube BioLegend's Fixation Buffer (Cat. No. 420801) at room temperature, in the dark, for 20 minutes. 3. Centrifuge at 350 x g for 5 minutes; discard supernatant. 4. Resuspend the cells in 0.5-1 mL/tube Cyto-Last™ Buffer, mix, cap the tubes, and then store at 4°C in the dark (Note: Cyto-Last™ Buffer can also be used for preserving cells in bulk at a cell concentration of 0.5-2.0 x 106 cells/mL.). 5. Take out the tubes at desired time points. Remove Cyto-Last™ Buffer by centrifugation, permeabilize the cells with BioLegend's Permeabilization Wash Buffer (Cat. No. 421002), and perform intracellular cytokine staining. |
| Product Citations | Parveen S, et al. 2023. Nat Commun. 7427:14. PubMed |
| Gene ID | NA |
| Regulatory Status | RUO |
| Other Names |
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| CytoLast Buffer |
