Biolegend, 422605, Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads
VO23
Catalog Number: 422605
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| Reactivity | Human |
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| Formulation | Beads are resuspended in phosphate buffer solution containing 0.1% recombinant HSA. |
| Endotoxin Level | < 1 EU/mL |
| Preparation | The antibodies were purified by affinity chromatography and conjugated with magnetic beads under optimized conditions. The final product is a GMP, ready to use solution. |
| Concentration | 4.0 x 107 ± 10% beads/mL |
| Storage & Handling | 4°C (2-8 °C) |
| Application | Cell Culture - Quality tested Activ - Verified in house |
| Recommended Usage | GMP CD3/CD28 Human T Cell activation beads should be plated with cells at a 1:1 bead-to-cell ratio. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes |
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| This product is designed for the in vitro or ex vivo expansion of human T cells. Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads are a ready to use solution. There is no need to dilute the product prior to use in cell culture. Suggested culture protocol: Prepare T Cell Complete Media (TCCM) using sterile, filtered IMDM with 5% final concentration of serum and 200 IU/mL of human IL-2. Prepare cells at a concentration of 1 x 106 per mL of TCCM. Wash 25 µL of Cell-Vive™ GMP CD3/CD28 Human T Cell Activation Beads to a 5 mL polypropylene tube with 1 mL TCCM. If using a 1:1 bead-to-cell ratio, wash 25 µL of beads per sample. Adjust the volume of beads depending on final bead-to-cell ratio. For isolated T cells, we recommend using a lower bead-to-cell ratio. 1:3 or 1:4 is recommended. Apply tube containing beads to a magnet (MojoSort magnet, cat#: 480019) for 2 minutes. Decant supernatant and remove tube from magnet. Resuspend beads in 1 mL of cell suspension and plate in a 12 well cell culture plate. Allow cells to expand at 37°C and 5% CO2. At Day 4 and Day 7, count cells and resuspend at 0.5 x 106 cells per mL. Harvest cells at Day 10 or Day 11 for further analysis. This product was optimized on isolated CD3+ T cells and PBMCs. Alternate sources of T cells may require protocol optimization. |
| Disclaimer | BioLegend Cell-Vive™ GMP Activation and Expansion tools are for research use only. Suitable for ex vivo cell processing use. Not for use in diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. |
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| Gene ID | NA |
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