Biolegend, 422608, Human CD3/CD28/CD137 T Cell Activation Beads

The Human CD3/CD28/CD137 T Cell Activation Beads allow for convenient in vitro activation of human T cells without the need for antigen-presenting cells or precoating culture plates. Human T cells are stimulated with microbeads coated with monoclonal anti-CD3, anti-CD28 and anti-CD137 antibodies. The antibodies present on the bead surface act as an agonist and stimulate T cells. This mimics antigen presenting cells to induce the proliferation, especially for memory T cells which rely on CD137 signaling for cell maintenance and activation. Activation in vitro with this product results in robust T cell expansion, especially of the central memory (CCR7 + CD45RA - ) CD8 + T cell subset. Simply add the beads directly to isolated T cells at a 1:1 particle-to-cell ratio for robust T cell activation. The antibody-coated beads can be removed from the cells using a magnetic separator such as the MojoSort™ Magnet (Cat. Nos. 480019, 480020, and 480173). Some of the downstream applications include functional assays, gene expression analysis, and phenotypic characterization.