Biolegend, 423001, Stop Solution for TMB Substrate
60ml
Catalog Number: 423001
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| Preparation | This solution is ready to use and requires no dilution. |
|---|---|
| Storage & Handling | Store at room temperature. |
| Application | ELISA |
| Application Notes | Each lot of Stop Solution for TMB (3,3'5,5'-tetramethylbenzidine) Substrate has been tested by ELISA. The TMB stop solution will stop TMB and HRP (horseradish peroxidase) reaction by adding the same volume as the TMB substrate added to each well. Addition of stop solution changes the color from blue to yellow. Read absorbance at 450 nm within 60 minutes. If the reader can read at 570 nm, the absorbance at 570 nm can be subtracted from the absorbance at 450 nm. |
| Additional Product Notes | Caution: Caustic material, strong acid. Wear eye, hand, and face protection. |
| Application References(PubMed link indicates BioLegend citation) | Kiianitsa K, et al. 2014. Nucleic Acids Res. 42:108. PubMed |
| Product Citations | Sinani G, et al. 2017. Int J Pharm. 10.1016/j.ijpharm.2017.06.053. PubMed Maizels K 2014. Nucleic Acids Res. 42:108. PubMed |
| RRID | SCR_001134 (BioLegend Cat. No. 423001) |
| Gene ID | NA |
| Regulatory Status | RUO |
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