Biolegend, 423302, Cell Activation Cocktail (without Brefeldin A)
400microl
Catalog Number: 423302
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Category
Pack Size4 vials
| Preparation | Cell Activation Cocktail (without Brefeldin A) is composed of PMA and ionomycin. |
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| Concentration | Each vial of this cocktail contains phorbol-12-myristate 13-acetate 40.5 µM (25 ug/mL) and ionomycin 669.3 µM (500 ug/mL) in DMSO (500X). |
| Storage & Handling | Store cocktail at -70°C upon receipt. Protect from light. Avoid repeat freeze/thaw cycles. |
| Application | Activ, FC, ICFC - Quality tested |
| Recommended Usage | Cat. No. 423301 contains 100 µL of cocktail, formulated at 500X, that can be used for activating 50 mL of cells of various types. Cat. No. 423302 contains 400 µL (4 vials x 100 µL per vial) of cocktail, formulated at 500X, that can be used for activating 200 mL of cells of various types. |
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| Application Notes |
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| Cell Activation Protocol: 1. Resuspend cells of interest in desired cell culture medium. Recommended cell suspension is between 1-2 x 106 cells/mL, although effective activation can be obtained with higher or lower concentration. 2. Completely thaw vials in 37°C water bath. 3. Add 2 µL of the cocktail to each mL of cell suspension. 4. Incubate cells at 37°C in a CO2 incubator for 6 hours or the time period of interest. 5. Harvest activated cells and centrifuge at 350g for 5 minutes. Discard supernatant. 6. Add 2.5 mL of Cell Staining Buffer (Cat. No. 420201) to the cell pellet, mix by vortex or pipetting, then centrifuge at 350g for 5 minutes. Discard supernatant. 7. Repeat step 6. 8. Cells are now ready to be tested for surface markers or proteins of interest. Note: If investigators are interested in detecting intracellular proteins following activation, this product can be used in conjunction with Monensin (Cat. No. 420701) or Brefeldin A (Cat. No. 420601). This product is temperature sensitive; repeated freeze/thaw is not recommended. Intracellular and surface flow staining, as well as ELISA, can be performed following activation of cells with this cocktail. |
| Additional Product Notes | View more applications data for this product in our Scientific Poster Library. |
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| Product Citations | Freimer JW, et al. 2022. Nat Genet. 54:1133. PubMed Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed Zhou Y, et al. 2023. Front Immunol. 14:1129705. PubMed Hu Y, et al. 2023. Front Immunol. 14:1128423. PubMed Gary EN, et al. 2023. Front Immunol. 14:1138609. PubMed Jung HS, et al. 2023. iScience. 26:106621. PubMed Malamba-Banda C, et al. 2023. Sci Rep. 13:9001. PubMed Chen J, et al. 2022. Adv Healthc Mater. 11:e2101714. PubMed Matiza T, et al. 2021. Medicine (Baltimore). 100:e28328. PubMed Kuloglu ZE, et al. 2022. Allergy. 77:2459. PubMed Mansouri S, et al. 2022. J Immunol. 209:2114. PubMed Tran TA, et al. 2023. Front Immunol. 13:1009484. PubMed Nanishi E, et al. 2023. NPJ Vaccines. 8:18. PubMed Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed Carpenter RS, et al. 2019. Sci Rep. 9:19105. PubMed McLean BA, et al. 2021. JCI Insight. 6:. PubMed Ayenehdeh J, et al. 2017. Immunology Letters. 10.1016/j.imlet.2017.05.006. PubMed Vazquez J, et al. 2020. Front Immunol. 2.545138889. PubMed Nagle VL, et al. 2022. Mol Cancer Ther. 21:658. PubMed Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed Cai B, et al. 2021. Mol Cancer. 20:165. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed Stephenson E, et al. 2021. Nat Med. 27:904. PubMed Qi X, et al. 2019. Nat Med. 25:1225. PubMed Lee J, et al. 2020. Sci Rep. 10:17753. PubMed Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed Uddin MN, et al. 2022. Brain Behav Immun Health. 26:100563. PubMed Owyong M, et al. 2019. Life Sci Alliance. 2:e201800226. PubMed Kumar RK, et al. 2021. Vascul Pharmacol. 138:106838. PubMed Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed Einkauf KB, et al. 2022. Cell. 185:266. PubMed Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed Michoud F, et al. 2020. Nat Biotechnol. . PubMed FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed Trial J, et al. 2020. Geroscience. . PubMed Spencer AJ, et al. 2022. EBioMedicine. 77:103902. PubMed Wang F, et al. 2021. Neoplasia. 23:281. PubMed Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Cell Stimulation Cocktail, PMA/Ionomycin |
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