Biolegend, 424001, DRAQ7™
1ml
Catalog Number: 424001
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| Preparation | DRAQ7™ is supplied at 1 ml per vial (300 µM). |
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| Storage & Handling | DRAQ7™ should be stored between 2°C and 8°C upon receipt. |
| Application | FC - Quality tested |
| Recommended Usage | DRAQ7™ is a trademark of Biostatus Limited. |
| Application Notes | DRAQ7™ is a membrane impermeable dye that only stains the nuclei of dead or permeabilized cells. It is a far-red emitting dye with optimal excitation/ emission peaks of 633nm/ 695nm, respectively. Suboptimally, DRAQ7™ can be excited at 561nm and 594nm as well, with the same emission peak occurring at 695nm. Ideally, this probe should be detected in the Alexa Fluor® 700 and APC/Cyanine7 emission filters off of the 633nm laser. Protocol for Dead Cell/Apoptosis Analysis using DRAQ7™: 1. Perform surface staining or Annexin V staining following protocol of choice. 2. Wash cells twice with phosphate buffered saline (PBS). Sodium azide interferes with DRAQ7™ staining; therefore it is recommended to stain in PBS (without calcium or magnesium) or in culture medium without sodium azide. 3. Dilute DRAQ7™ to your required concentration. We recommend titrating the reagent to determine optimal concentration for cells of interest. Good results have been observed using a 1:200 to 1:1000 dilution. 4. Incubate at room temperature, preventing exposure to light, for 10-15 minutes. No washing is necessary. 5. Analyze cells on a cytometer equipped with the appropriate laser. |
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| Product Citations | Pavesi A, et al. 2016. Sci Rep. 6: 26584. PubMed Garvey C, et al. 2016. Sci Rep. 6:29752. PubMed |
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| Biology Area | Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Neuroscience |
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| Antigen References | 1. Akagi J, et al. 2013. Cytometry A. 83: 227. 2. Smith PJ, et al. 2013. Cytometry A. 659-71. 3. Wlodokowic D, et al. 2013. Curr Protoc Cytom. Chapter 9. Unit 9.4.1 |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Live/Dead Staining, Dead cell exclusion dye, Cell viability dye |
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