Biolegend, 424601, Compensation Beads
25tests
Catalog Number: 424601
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| Formulation | Each kit will contain sets of two dropper bottles as listed below: 1. Negative beads 2. Positive beads |
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| Preparation | Compensation Beads are supplied ready to use, 1 drop (~25-30 µL) from each bottle is sufficient for one test. |
| Storage & Handling | Store the kit at 2°C - 8°C upon receipt. |
| Application | FC - Quality tested |
| Recommended Usage | 1 drop of positive beads plus one drop of negative beads |
| Application Notes | Compensation Beads can bind mouse, rat, rabbit, donkey, hamster and human monoclonal or polyclonal antibodies. Antibodies from other host species have not been verified to bind to the positive beads and may not be compatible for use with this product. Product is a kit of one positive beads bottle and one negative beads bottle. Compensation Beads can be used as compensation controls for multicolor flow cytometry assays or in any other assay that require antibody binding beads. Protocol for using Compensation Beads: Vortex beads vigorously for 1-2 minutes prior to using to disrupt aggregates that may have formed during storage. Prepare tubes with appropriate antibodies (single color). Add one drop of beads from each bottle (positive and negative). Mix thoroughly. Ensure that any stray liquid on the side of the tube is mixed in with the antibody. Incubate at room temperature for 15-20 minutes, protected from exposure to light. Wash beads with Cell Staining Buffer (Cat. No. 420201) or equivalent. Centrifuge at 350Xg for 5 minutes and re-suspend in Cell Staining Buffer or equivalent. Beads are ready to acquire. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Compensation, Comp Beads |
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