Biolegend, 424701, Deep Blue Cell Viability™ Kit

Name: Deep Blue Cell Viability™ Kit
Brand: Biolegend
SKU: 424701

25ml

Catalog Number: 424701

Pricing

Formulation	Aqueous solution.
Preparation	The solution is filtered through a 0.22 µm membrane that contains no preservatives.
Storage & Handling	Store at 2°C to 8°C for up to 12 months.
Application	FA - Quality tested
Recommended Usage	Add 10% of the Deep Blue Cell Viability™ Kit to the wells containing the cells to study. For example: in a well containing 100 µL of cell culture, add 10 µl of the Deep Blue Cell Viability™ reagent. Incubate as needed depending on the cell type, which is typically one - six hours. If visible crystals are observed, filter the reagent through 0.22 µm filter prior to use.
Application Notes	The Deep Blue Cell Viability™ Kit is based on the resazurin reagent. The typical blue color of resazurin is converted to fluorescence by the action of metabolic enzymes. The reduction of resazurin into resorufin can be detected using a fluorometer (Excitation: 530-570 nm, Emission = 590-620 nm).
Application References(PubMed link indicates BioLegend citation)	Nakayama GR, et al. 1997. J. Immunol. Methods. 2:205. Wilson I, et al. 2000. Eur. J. Biochem. 17:5421.

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