Biolegend, 425201, Calcein-AM
10x50microg
Catalog Number: 425201
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Category
Pack Size10 vials
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| Preparation | Calcein-AM labeling kit consists of lyophilized Calcein-AM and anhydrous DMSO. For reconstitution, bring the kit to room temperature and add 50 µL of DMSO to one vial of Calcein-AM dye until fully dissolved. |
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| Storage & Handling | Upon receipt, Calcein-AM should be stored at -20°C. Do not open vials until needed. Once the DMSO is added to the Calcein-AM, use immediately or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than one month. |
| Application | FC - Quality tested |
| Recommended Usage | This lot has been tested by flow cytometric analysis of cell vitality. It can be used at concentrations ranging from 0.1 – 0.001 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application. |
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| Application Notes |
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| The molecular weight of Calcein-AM is 994 Da. The maximum excitation and emission wavelengths of Calcein-AM are 488 nm/520 nm, respectively. Each 50 µg of Calcein-AM may be reconstituted with 50 µL of anhydrous DMSO to yield a stock concentration of 1 mM. Materials Provided: 10 vials x 50 µg Calcein-AM 2 vials of 500 µl anhydrous DMSO Calcein-AM Labeling Procedure: 1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial. 2. Prepare a 1 mM stock solution by reconstituting 1 vial of lyophilized Calcein-AM dye with 50 µl of anhydrous DMSO. 3. Prepare a 1 µM working solution by diluting 1 µL of 1 mM Calcein-AM stock solution in 1 mL PBS. 4. Spin down cells and adjust the cell suspension to 1x107 cells/mL in PBS. 5. Add 10µl of the 1 µM Calcein-AM working solution to each mL of cell suspension for a final concentration of 0.01 µM. 6. Incubate cells for 20 minutes at room temperature or at 37°C and keep protected from light. 7. Pellet cells and resuspend in pre-warmed cell culture medium. 8. Incubate cells for 10 minutes to ensure optimal retention of the Calcein-AM. 9. After incubation, Calcein-AM labeled cells are ready for downstream applications or analysis. |
| Application References(PubMed link indicates BioLegend citation) | Mariappan MR, et al. 1999. IEEE Eng. Med. Biol. Mag. 18:22. Mironov SL, et al. 2005. J. Biol. Chem. 280:715. Tian F, et al. 2009. Blood. 113:5352. Ansbro MR, et al. 2013. PLoS One 8(4):e60334. (IF) Gonzalez JM, et al. 2013. Invest. Ophthalmol. Vis. Sci. 54:1039. (IF) Godin LM, et al. 2011. Am. J. Physiol. Lung Cell Mol. Physiol. 300:L615. (IF) |
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| Product Citations | Li S, et al. 2022. Elife. 11:. PubMed Abeynaike S, et al. 2023. Viruses. 15:. PubMed Bhatt RS, et al. 2021. Cancer Immunol Res. 9:156. PubMed Yongabi D, et al. 2022. Adv Sci (Weinh). 9:e2200459. PubMed Oude Egberink R, et al. 2022. Pharmaceutics. 14: . PubMed McNulty J, et al. 2023. Sci Rep. 13:1639. PubMed Yang F, et al. 2022. J Cell Mol Med. 26:2363. PubMed Chan JM, et al. 2021. Cancer Cell. 39:1479. PubMed Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed Wang K, et al. 2020. Sci Adv. 6:eaba7606. PubMed Riedelberger M, et al. 2020. Bio Protoc. e3552:10. PubMed Quintanal-Villalonga A, et al. 2022. STAR Protoc. 3:101776. PubMed Huang N, et al. 2020. Genome Biol. 1.03125. PubMed Huang RS, et al. 2021. Curr Protoc. 1:e246. PubMed Lavoie RR, et al. 2021. Cancers (Basel). 13:. PubMed Wachendörfer M, et al. 2022. Biomater Sci. :. PubMed Hedemann N, et al. 2021. Cancers (Basel). 13:. PubMed Feng Y, et al. 2022. Life (Basel). 12:. PubMed Rossignol A, et al. 2017. MAbs. 9:521. PubMed |
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| Structure | Acetoxymethyl ester. |
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| Distribution | Cytoplasmic. |
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| Function | Cell vitality indicator. |
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| Interaction | Nonspecific esterases. |
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| Biology Area | Cell Proliferation and Viability |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Calcein, Calcein AM |
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