Biolegend, 425203, Calcein Violet-AM
10x25microg
Catalog Number: 425203
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Category
Pack Size10 vials
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| Preparation | Calcein Violet-AM labeling kit consists of lyophilized Calcein Violet-AM and anhydrous DMSO. For reconstitution, bring the kit to room temperature and add 40 µL of DMSO to one vial of Calcein Violet-AM dye until fully dissolved. |
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| Storage & Handling | Calcein Violet-AM should be stored at -20°C upon receipt. Do not open vials until needed. Once the DMSO is added to the Calcein Violet-AM, use immediately or store at -20°C in a dry place and protected from light, preferably in a desiccator or in a container with desiccant for no more than one month. |
| Application | FC - Quality tested |
| Recommended Usage | This lot has been tested by flow cytometric analysis of cell vitality. It can be used at concentrations ranging from 0.1 – 0.001 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application. |
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| Application Notes |
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| The molecular weight of Calcein Violet-AM is 616.48 Da. The maximum excitation and emission wavelengths of Calcein Violet-AM are 400 nm/452 nm, respectively. Each 25 µg of Calcein Violet-AM may be reconstituted with 40 µL of anhydrous DMSO to yield a stock concentration of 1 mM. Materials Provided: 10 vials x 25 µg Calcein Violet-AM 2 vials of 500 ul anhydrous DMSO Calcein Violet-AM Labeling Procedure: 1. Prior to reconstitution, spin down the vial of lyophilized reagent in a microcentrofuge to ensure the reagent is at the bottom of the vial. 2. Prepare a 1 mM stock solution by reconstituting 1 vial of lyophilized Calcein Violet-AM dye with 40 µl of anhydrous DMSO. 3. Prepare a 1 µM working solution by diluting 1 µL of 1 mM Calcein Violet-AM stock solution in 1 mL PBS. 4. Spin down cells and adjust the cell suspension to 1x107 cells/mL in PBS. 5. Add 10 µl of the 1 µM Calcein Violet-AM working solution to each mL of cell suspension for a final concentration of 0.01 µM. 6. Incubate cells for 20 minutes at room temperature or at 37°C, protected from light. 7. Pellet cells and resuspend in pre-warmed cell culture medium. 8. Incubate cells for 10 minutes to ensure optimal retention of the Calcein Violet-AM. 9. After incubation, Calcein Violet-AM labeled cells are ready for downstream applications or analysis. |
| Application References(PubMed link indicates BioLegend citation) | Eastburn DJ, et al. 2014. Nucleic Acids Res. 42:e128. (FC) Zhou Q, et al. 2012. Blood. 120:4334. (FC) Fr÷hlich JD, et al. 2012. Am. J. Pathol. 180:153. Wang T, et al. 2012. ACS Nano. 6:1251. Lis R, et al. 2011. Int. J. Cancer 128:715. (FC) Cole LE, et al. 2007. Infect. Immun. 75:4127. (IF) |
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| Product Citations | Rommel MGE, et al. 2022. Cell Rep. 41:111447. PubMed |
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| Structure | Acetoxymethyl ester. |
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| Distribution | Cytoplasmic. |
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| Function | Cell vitality indicator. |
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| Interaction | Nonspecific esterases. |
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| Biology Area | Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Calcein, Calcien Violet-AM |
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