Biolegend, 426313, Western-Ready™ Transfer Buffer (10X)
1l
Catalog Number: 426313
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Send an RFQ for Biolegend 426313, Western-Ready™ Transfer Buffer (10X). Iright supports global sourcing and international shipment for life science and laboratory products.
| Formulation | Proprietary |
|---|---|
| Preparation | This buffer is bottled as 10X. To prepare a working solution (1X) dilute Western-Ready™ Transfer Buffer (10X) to 1X concentration (1:10 by volume). 1 part of Western-Ready™ Transfer Buffer (10X), 2 parts of 100% methanol, and 7 parts of DI water. |
| Storage & Handling | Upon receipt, store solutions at room temperature. Products are shipped at ambient (room) temperature. |
| Application | WB - Quality tested |
| Recommended Usage | Dilute Western-Ready™ Transfer Buffer (10X) to 1X concentration (1:10 by volume). 1 part of Western-Ready™ Transfer Buffer (10X), 2 parts of 100% methanol, and 7 parts of DI water. |
| Application Notes | This buffer is formulated for Western blot protein transfer. By direct PDVF membrane staining using Licor Revert 700 protein dye, we are able to detect as low as 25 ng/band on high and medium molecular weight proteins, and as low as 12.5 ng/band in low molecular weight proteins. The buffer is validated for protein transfer to both nitrocellulose and PVDF membranes. This buffer is only recommended for wet protein transfers. Western-Ready™ Transfer Buffer does not include any methanol. |
| Gene ID | NA |
| Regulatory Status | RUO |
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