Biolegend, 426504, Multiplex Immunohistochemistry Kit (3-color TSA)
250tests
Catalog Number: 426504
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| Verified Reactivity | Species independent |
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| Preparation | Please refer to the detailed protocol in the Related Protocols section. |
| Storage & Handling | Kit Contents (90/250 tests) 3% H2O2 (150/300 µL) - Store at 4°C Biotin Block Buffer (300/600 µL) - Store at 4°C HRP Block Buffer (9/25 mL) - Store at 4°C Block Activator (1450/1450 µL) - Store at 4°C StreptaClick®–HRP (170/500 µL) - Store at 4°C TSA Amplification Buffer Plus (25 mL) - Store at 4°C 30% H2O2 (100 µL) - Store at 4°C Tyramide 488 (80 µg) - Store at -20°C Tyramide 555 (80 µg) - Store at -20°C Tyramide 647 (80 µg) - Store at -20°C |
| Application | IHC-F - Quality tested IHC-P - Verfiied |
| Recommended Usage | Please see the detailed protocol below in the Related Protocols section. |
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| Application Notes |
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| This kit can be used for multiplex immunohistochemistry using Tyramide Signal Amplification (TSA) on frozen or formalin-fixed paraffin embedded (FFPE) tissue sections. The protocol does not use heat treatment between cycles, which also allows TSA multiplex immunostaining on frozen tissue sections. This kit uses click reaction to attach monovalent horseradish peroxidase (HRP) to your biotinylated antibody of choice. The HRP-labeled biotinylated antibodies can then be used for multiple cycles of immunostainings where each fluorochrome is sequentially developed by TSA. The kit contains an HRP Block Buffer optimized for preserving the morphology of frozen tissue sections. The HRP block buffer rapidly quenches the HRP enzyme after each TSA cycle and allows for sequential multiplex IHC. Please see the detailed protocol below: https://www.biolegend.com/en-us/protocols/multiplex-immunohistochemistry-kit-3-color-tsa-protocol Click here to view BioLegend’s biotin-labeled antibodies which are verified for use in immunohistochemistry. |
| Additional Product Notes | Name Absoption Max Emission Max Tyramide 488 494 nm 517 nm Tyramide 555 555 nm 572 nm Tyramide 647 649 nm 671 nm Tyramide Dyes: Fluorescence tyramide dyes enable tyramide signal amplification (TSA), delivering exceptional signal strength for immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH) applications. How TSA Works: TSA leverages horseradish peroxidase (HRP) chemistry to achieve dramatic signal enhancement. In the presence of hydrogen peroxide, HRP catalyzes the conversion of tyramide-containing dyes into highly reactive free radicals. These activated molecules covalently bind to tyrosine residues at or near the HRP location, creating permanent, amplified signals directly at your target site. |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Multiplex IHC Kit, Multiplex IHC TSA Amplification Kit, StreptaClick®–HRP |
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