Biolegend, 427201, Veri-Cells™ Heavy Metal (Ta) PBMC
10tests
Catalog Number: 427201
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Category
Pack Size2 vials
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| Formulation | Cat. No. 427201: 2 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC).Cat. No. 427203: 10 vials each containing ≥ 1 X 106 lyophilized human peripheral blood mononuclear cells (PBMC). |
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| Preparation | The Veri-Cells™ Heavy Metal Ta PBMC kit is prepared from 2 vials of lyophilized human PBMCs labeled with tantalum and a vial of Veri-Cells™ Buffer A Plus. |
| Storage & Handling | Store the kit at 2°C - 8°C upon receipt. Do not open sealed pouch until ready to use. Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided. |
| Application | FC, CyTOF® - Quality tested |
| Recommended Usage | 50 µL per test (after reconstitution with 325 µL of Veri-Cells™ Buffer A Plus) Once reconstituted, let the cells sit for 15 minutes at room temperature prior to staining. Veri-Cells™ Buffer A Plus may show precipitation over time, however this is normal and does not affect performance of the buffer. |
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| Application Notes |
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| The Veri-Cells™ Heavy Metal Ta PBMC kit contains stabilized human blood cells which have been pre-labeled with the metal tantalum (Ta). The reconstituted human PBMCs can be added to test samples as a control for CyTOF applications. The cells have been verified to work with commonly tested cell markers such as CD3, CD4, CD8, CD14, CD16, CD19, CD38, CD56, CD123 and HLA-DR. Assessing the suitability of this product as a control for the end user’s molecule of interest is recommended. The tantalum signal is confirmed by a CyTOF Helios or equivalent mass cytometry instrument. Cell subset Acceptance Criteria, % positive of lymphocyte gate CD3+ 55-95 CD4+CD3+ 30-65 CD8+CD3+ 10-40 CD19+CD3 – 3-30 CD56+CD16+CD3 – 3-40 Note on indicated ranges: Percent positive values are obtained based on gates set on unstained cells. The antibodies used for testing were the following: CD56 PE (clone 5.1H11), CD16 PE (clone 3G8), CD19 APC (clone HIB19), CD3 FITC (clone UCHT1), CD4 PE/Cyanine7 (clone RPA-T4), and CD8 APC/Cyanine7 (clone RPA-T8). Variability in antibodies used, instruments, staining conditions and other factors may influence end-user values. The Certificate of Analysis specific values are provided as a reference. It is recommended that individual laboratories establish its own control values and ranges for each parameter based on laboratory specific conditions and protocols. Please use our CoA Look-Up Tool to find the expected lot-specific frequencies of certain cell populations included within each lot of Veri-Cells™ Heavy Metal (Ta) PBMC. |
| Additional Product Notes | Veri-Cells™ Buffer A Plus is an improved version of the original Veri-Cells™ Buffer A to improve staining performance with tandem dyes. |
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| Product Citations | Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed Lee HJ, et al. 2023. iScience. 108387:26. PubMed |
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| Gene ID | NA |
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| Regulatory Status | RUO |
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| Other Names | Control cells |
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