Biolegend, 431324, ELISpot MAX™ Deluxe Set Mouse IL-6

Mouse IL-6 (Interleukin-6) is a multifunctional cytokine known by various names such as B-cell Stimulatory Factor 2 (BSF2) and Hybridoma Growth Factor (HGF). It is produced by a variety of cells including macrophages, T-cells, osteoblasts, endothelial cells, and hepatocytes. IL-6 plays a pivotal role in both pro-inflammatory and anti-inflammatory responses. It is crucial in numerous biological processes such as B-cell maturation, osteoclast formation, nerve cell differentiation, muscle healing, and muscle hypertrophy. Dysregulation in the expression and activity of Mouse IL-6 can lead to many diseases, including chronic inflammation, cancer, and neurodegenerative disorders. BioLegend’s ELISpot MAX™ Deluxe Set contains pre-optimized essential reagents and additional buffers and solution for Enzyme-Linked Immunosorbent Spot assay. This kit is designed for detection of mouse IL-6 secreting cells. An antibody specific for mouse IL-6 is coated on ethanol-activated 96-well PVDF membrane plate as the capture. Stimulated and/or unstimulated cells are pipetted directly into the wells and then incubated at 37˚C for a specific period of time. During this period, capture antibody binds to mouse IL-6 from the secreting cells in the vicinity. Then, cells are removed, and a biotinylated anti-mouse IL-6 detection antibody is added, followed by avidin-HRP. Subsequently, TMB membrane substrate is added. The TMB membrane substrate reacts with HRP to form a blue-black precipitate at the sites of mouse IL-6 secreting cells. The result is visible spots, which corresponds to footprints of mouse IL-6 secreting cells, on the membrane surface.