Biolegend, 433927, LEGEND MAX™ High Sensitivity Human IL-17A ELISA Kit

Interleukin 17A (IL-17A), also known as IL-17 or CTLA-8, is a proinflammatory cytokine essential for immune defense, inflammation, and tissue pathology. It signals through a receptor complex of IL-17RA and IL-17RC, activating NF-κB and MAPK pathways, which drive the expression of cytokines, chemokines, and antimicrobial peptides. IL-17A is primarily produced by CD4+ Th17 cells, but also by CD8+ T cells, γδ T cells, and innate lymphoid cells. It plays a key role in the pathogenesis of autoimmune diseases, and IL-17A-targeted therapies are being developed for chronic inflammatory disorders. The BioLegend LEGEND MAX™ High Sensitivity Human IL-17A ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal anti-human IL-17A capture antibody. The detection antibody is a biotinylated polyclonal anti-human IL-17A antibody. After washing away any unbound biotinylated detection antibody, a streptavidin-polymer HRP is used for detection. Streptavidin-Polymer HRP enhances the sensitivity of the kit, allowing for detection of low concentrations of IL-17A. This kit is specifically designed for the accurate quantitation of human IL-17A in serum, heparin plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.