Biolegend, 436017, LEGEND MAX™ Human Tau (x-421) ELISA Kit

Tau is a 441-amino acid protein that binds along microtubules in order to stabilize them. Phosphorylation of tau reduces its affinity for microtubules, leading to microtubule destabilization. Hyperphosphorylated tau can assemble into higher order structures, including oligomers and neurofibrillary tangles (NFT). NFTs are a hallmark of several neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and frontotemporal dementia. In addition to phosphorylation, proteolytic cleavage of tau is an important post-translational modification. For example, caspase-3-mediated cleavage of tau at Asp421 is an early step in NFT formation and has been linked to an increase in neurotoxicity in AD patients. Furthermore, the number of NFTs composed of tau (x-421) is positively correlated with AD progression. Tau cleavage products have also been found in PD patients, thus demonstrating that truncated forms of tau could be a biomarker for multiple neurodegenerative diseases. The BioLegend LEGEND MAX™ Human Tau (x-421) ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a monoclonal mouse anti-human tau (x-421) capture antibody. The detection antibody is a biotinylated monoclonal mouse anti-human tau antibody. This kit is specifically designed for the accurate quantitation of human tau (x-421) in human cell/tissue lysates. This kit is analytically validated with ready-to-use reagents.