Biolegend, 438205, ELISA MAX™ Deluxe Set Rat TNF-a

Please contact our sales representative for bulk pricing quotations. TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing.  Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro , hemorrhagic necrosis of tumors in vivo , increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.  It has been shown that TNF-α plays a critical role in many physiological and pathological conditions including neurodegenerative diseases and cancer. This kit is specifically designed for the accurate quantitation of Rat TNF-α from cell culture supernatant, serum, plasma, or other biological fluids.  It is designed to meet the increasing use of rat models in biomedical studies.