Biolegend, 439209, RAPID MAX™ Human Granzyme B ELISA Kit

Human Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer (NK) cells. Human Granzyme B is one of the five members of the human granzyme family which includes Granzymes A, B, G, H, and K. Granzyme B is expressed as a 32 kD protein encoded by the GZMB gene. While Granzyme B is mostly expressed by CTL (Cytotoxic T Lymphocyte) and NK cells, it is also expressed in resting and activated plasmacytoid dendritic cells. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. Granzyme B and perforin have been shown to induce CTL-mediated target cell DNA fragmentation and apoptosis. Once released from the CTL, Granzyme B binds its receptor, the mannose-6-phosphate/insulin-like growth factor II receptor, and is endocytosed but remains arrested in endocytic vesicles until released by perforin. Once in the cytosol, Granzyme B targets caspase-3 and other caspases directly or indirectly through the mitochondria, initiating the caspase cascade which leads to DNA fragmentation and apoptosis. Elevated Granzyme B levels have been linked with various disease processes. The BioLegend RAPID MAX™ Human Granzyme B ELISA Kit is a one step, 75-minute enzyme-linked immunosorbent sandwich assay (ELISA) that includes a 96-well strip plate that is pre-coated with an anti-tag antibody. Standards and samples are added into the wells, followed by an antibody cocktail. The antibody cocktail consists of a tag-conjugated capture antibody and an HRP-conjugated detection antibody. This kit is specifically designed for the accurate quantitation of human Granzyme B cell culture supernatant. This kit is analytically validated with ready-to-use reagents