Biolegend, 443208, LEGEND MAX™ Mouse PCSK9 ELISA Kit

Protein Convertase Subtilisin/Kexin Type 9 (PCSK9) belongs to the proteinase K subfamily of subtilases and is expressed as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. Mouse PCSK9 is a 694 amino acid glycoprotein and shares 78% and 92% amino acid identity with human and rat PCSK9, respectively. PCSK9 is mainly expressed in liver, and at lower levels in kidney, intestine, and brain. It regulates plasma cholesterol homeostasis via binding to low-density lipid receptor family members such as Low-density lipoprotein receptor (LDLR). The binding of PCSK9 to LDLR enhances the degradation of the LDLR in endosomes/lysosomes, resulting in increased circulating LDL-Cholesterol (LDL-C). Elevated LDL-C levels are a major risk factor for cardiovascular disease and atherosclerosis. Individuals with genetic loss-of-function mutations in PCSK9 have reduced plasma levels of LDL-C and are protected from coronary heart disease. Thus, PCSK9 has gained attention as a pharmacological target for cardiovascular diseases and atherosclerosis. BioLegend's LEGEND MAX™ Mouse PCSK9 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat anti-mouse PCSK9 monoclonal antibody. The Detection Antibody is a Biotinylated goat anti-mouse PCSK9 polyclonal antibody. This kit is specifically designed for the accurate quantitation of mouse PCSK9 from serum, plasma, cell culture supernatant, cell lysate, tissue homogenate, and other biological fluids. It is analytically validated wtih ready-to-use reagents.