Biolegend, 444307, LEGEND MAX™ Human Leptin ELISA Kit

Human Leptin is a 167 amino acid protein and approximately 16 kD in mass. Leptin is encoded by the obese (ob) gene and the gene locates on chromosome 7. Leptin is an adipocyte-derived hormone and mainly secreted by adipocyte. Small amounts of Leptin are expressed by the cells in the epithelium of the stomach and in the placenta. Leptin’s effects on body weight are mediated through impacts on hypothalamic center that control feeding behavior, hunger, body temperature, and energy expenditure. Leptin importantly regulates food intake, body weight, metabolism, energy expenditure, reproductive function, and adiposity through the hypothalamic Leptin receptor. Leptin is also the member of long-chain helical cytokine family. The BioLegend LEGEND MAX™ Human Leptin ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human Leptin antibody. The detection antibody is a biotinylated mouse monoclonal anti-human Leptin antibody. This kit is specifically designed for the accurate quantitation of human Leptin in serum, plasma, and cell culture supernatant. This kit is analytically validated with ready-to-use reagents.