Biolegend, 446607, LEGEND MAX™ Human BDNF ELISA Kit

Brain-derived neurotrophic factor (BDNF) is the second member of NGF family of neurotrophic factors (neurotrophins), which includes nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammalian species examined to date. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development. BDNF participates in axonal growth, pathfinding, and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the central nervous system (CNS). High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor. BDNF is also associated with multiple diseases such as Schizophrenia, Rett syndrome, obsessive compulsive disorder, Huntington’s disease, eating disorders, depression, dementia and Alzheimer’s disease. For example, BDNF level is decreased in Alzheimer’s disease patients’ serum samples. The BioLegend LEGEND MAX™ Human BDNF Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human BDNF capture antibody. The detection antibody is a biotinylated mouse monoclonal anti-human BDNF antibody. This kit is specifically designed for the accurate quantitation of the free-form human BDNF from cell culture supernatant, serum, plasma, and other biological fluids. It has been analytically validated with ready-to-use reagents.