Biolegend, 446904, ELISA MAX™ Deluxe Set Mouse CCL8 (MCP-2)

Note: This product does not include ELISA microwell plates. Cat. No. 423501 is recommended. CCL8, also known as MCP-2, is a CC chemokine of the monocyte chemoattractant protein (MCP) family that plays key roles in allergic and inflammatory responses. CCL8 (MCP-2) has structural and functional similarity to other family members MCP-1 and MCP-3, and is often co-expressed with these chemokines in mononuclear cells. CCL8 is also constitutively expressed in the skin and lymph nodes of mice. Mouse CCL8 induces a chemotactic migration of a population of differentiated Th2 cells enriched in IL-5 and IL-25 receptors by binding to the CCR8 receptor. Recent studies have established CCL8 as a critical ligand for driving CCR8-dependent Th2 cell trafficking into the skin, which in conjunction with locally recruited eosinophils drives chronic cutaneous allergic inflammation. CCL8 expression by suprabasal buldge cells has also been experimentally shown to negatively regulate the population of mouse hair follicles by Langerhans cells, further underscoring this chemokines central contributions to maintaining cutaneous immunity. The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The mouse (CCL8 MCP-2) capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and CCL8 (MCP-2) binds to the immobilized capture antibody. Next, biotinylated anti-mouse CCL8 (MCP-2) detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of CCL8 (MCP-2) present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.