Biolegend, 447207, LEGEND MAX™ Flex-T™ Human Class I Peptide Exchange ELISA Kit

BioLegend’s Flex-T™ MHC products allow high flexibility in studying antigen specific T cells. Biotinylated MHC monomers are manufactured with a UV-labile peptide that can be degraded with an ultraviolet light source. This allows for a peptide exchange when UV irradiation is performed in the presence of a peptide of interest (shown in below graph). This flexibility permits the screening of virtually any peptide of interest with enough affinity for the MHC allele that it is loaded onto. Refer to the BioLegend Flex-T website for more details ( www.biolegend.com/en-us/flex-t ). BioLegend’s LEGEND MAX™ Flex-T™ HLA Class I ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human β2-microglobulin antibody. HRP-conjugated streptavidin is the detection reagent that binds to biotinylated HLA class I monomer. Through binding of β2-microglobulin subunit and biotin conjugated on the HLA class I monomer, this Sandwich assay detects the HLA class I α-chain/β2-microglobulin/peptide complex and only intact complex is recognized. Therefore, exchange product with high affinity peptides will show relatively high signals in this assay, while moderate or low affinity peptides exchange would result in relatively lower or non-detectable signals. A Flex-T™ HLA Class I ELISA Control is included in the kit as the assay control. This kit is not intended to be used to measure the actual affinity of peptides, but is specifically designed to qualitatively evaluate the efficiency of UV peptide exchange when loading peptides of interest into Flex-T™ HLA class I monomers. Each assay kit comes with analytically validated, ready-to-use reagents.