Biolegend, 449604, ELISA MAX™ Deluxe Set Human Phospho-EGFR (Tyr1148)

EGFR (Epidermal Growth Factor Receptor) is a member of the ErbB/HER family of cell surface receptors which comprises of four closely related tyrosine kinase receptors: EGFR/HER1, HER2, HER3, and HER4. Binding of a ligand to the extracellular domain of EGFR results in receptor dimerization and autophosphorylation on tyrosine residues with the major phosphorylation sites being tyrosine 1068 (Tyr1068), Tyr1148, and Tyr1173. Phosphorylation of Tyr1148 leads to interaction with Shc and phospholipase Cγ (PLCγ), which are involved in the activation of MAPK signaling pathway. EGFR activation triggers intracellular signaling that results in increased proliferation and a number of processes related to cell survival. ErbB receptors are overexpressed or mutated in many cancers. It was reported that deficient signaling of the EGFR can result in degenerative diseases such as Alzheimer’s disease. BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant human Phospho-EGFR (Tyr1148). The set is designed for cost-effective and accurate quantification of human Phospho-EGFR (Tyr1148) in cell and tissue lysate. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.