Biolegend, 480003, MojoSort™ Mouse Hematopoietic Progenitor Cell Isolation Kit

Mouse hematopoietic stem/progenitor cells are enriched by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched hematopoietic stem/progenitor cells are collected by decanting the liquid in a clean tube. These are your cells of interest; do not discard the liquid. The enriched cells can be used for downstream applications including functional assays, gene expression, phenotypic characterization, etc. MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend. Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.