Biolegend, 480038, MojoSort™ Human CD4 T Cell Selection Kit

In this two-step kit, monocytes are first depleted by incubating PBMCs with biotin anti-human CD14 antibody, followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator; the unlabeled fraction is collected in a clean tube. In the second step, CD4 + T cells are positively selected from the previous unlabeled fraction by incubating it with directly conjugated Human CD4 Nanobeads. The second magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD4 + T cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend. Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.