Biolegend, 480042, MojoSort™ Human CD4 Naïve T Cell Isolation Kit

Name: MojoSort™ Human CD4 Naïve T Cell Isolation Kit
Brand: Biolegend
SKU: 480042

200tests

Catalog Number: 480042

Pricing

Verified Reactivity	Human
Formulation	Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation	The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling	Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application	Cell Separation (MojoSort™) - Quality tested
Recommended Usage	10 µl of antibody cocktail for 1 x 10^7 cells in 100 µl of buffer. 10 µl of Streptavidin Nanobeads for 1 x 10^7 cells in 100 µl of buffer.
Application Notes	This kit is designed for the isolation of untouched naïve CD4+ T cells from peripheral blood mononuclear cells (PBMCs). This kit is designed for the MojoSort™ Isolation Kits No Wash Protocol. However, if one wash is added after the incubation with Streptavidin Nanobeads the purity increases about 2 - 4%. Typically without the wash the purity ranges from 91 - 96%. With one wash after the Streptavidin Nanobeads incubation the purity ranges from 93 - 98%. Purity is reported relative to target cells not previously gated on CD4+ cells. Each lot has been individually optimized. Do not mix and match components from different lots or different kits.

Ordering

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