Biolegend, 480099, MojoSort™ Mouse CD3 Selection Kit

Mouse CD3 + cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse CD3 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD3 + expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Clone 17A2 recognizes ε/γ (but not ε/δ) of the CD3 complex. It can induce T cell activation. MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend. Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.