Biolegend, 480171, MojoSort™ Mouse CD146 (LSEC) Selection Kit

Mouse CD146+ liver sinusoidal endothelial cells are either selected or depleted by incubating your sample with the anti-mouse CD146 Antibody Cocktail followed by the Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the labeled CD146+ LSECs of interest; do not discard. After collection of the CD146+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend. Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.