Biolegend, 480183, MojoSort on Columns™ - Human CD4 T Cell Isolation Kit
100tests
Catalog Number: 480183
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| Verified Reactivity | Human |
|---|---|
| Formulation | Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2. Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. |
| Preparation | The antibodies were purified by affinity chromatography and conjugated with biotin under optimal conditions. Streptavidin Nanobeads: Streptavidin-coated magnetic beads |
| Storage & Handling | Antibody cocktail and Streptavidin Nanobeads should be stored upright and undiluted between 2°C and 8°C. For lot-specific expiration dates, please refer to the Certificate of Analysis which can be found here. |
| Application | Cell Sep – Neg (Quality Tested) |
| Recommended Usage | 10 µL of antibody cocktail for 1 x 107 cells in 100 µL of buffer. 10 µL of Streptavidin Nanobeads for 1 x 107 cells in 100 µL of buffer. |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Application Notes |
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| This kit is designed for negative isolation of CD4+ T cells from human PBMCs derived from whole blood or leukopaks. This kit is optimized for use with a column-based separation system such as MojoSort on Columns™ - Large Columns (Cat. No. 480178), Multistand (Cat. No. 480177) and Column Separator (Cat. No. 480179). This kit has also been tested for compatibility with columns from other suppliers. Please contact BioLegend Technical Service ([email protected]) for more information and guidance on compatibility with 3rd party columns and column systems. This product is designed research use only (RUO). Important Note: This kit is not suitable for use with our handheld magnets. If you're using handheld magnets, we recommend the MojoSort™ Human CD4 T Cell Isolation Kit (Cat. No. 480009). Test sizes/starting cell capacity: One test is defined as cell separation from 1x107 human whole blood PBMCs or leukopak-derived PBMCs. The 100 tests size provides enough reagents for approximately 1x109 cells. Each lot has been individually optimized. Do not mix and match components from different lots of different kits. |
| Gene ID | NA |
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| Regulatory Status | RUO |
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