Biolegend, 480199, MojoSort™ Human CD63 Microbead Exosome Kit
1kit
Catalog Number: 480199
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| Verified Reactivity | Human |
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| Formulation | Particle: Aqueous solution containing sodium phosphate Buffer: Aqueous solution |
| Preparation | The antibodies were purified by affinity chromatography and conjugated onto the magnetic carboxyl microbeads. |
| Storage & Handling | All components should be stored upright and undiluted between 2°C and 8°C. |
| Application | Purification – Quality tested |
| Recommended Usage | 20 μL of Microbeads per 0.5 mL human biofluids (serum, heparin-treated plasma, cell culture supernatant or urine) |
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| Application Notes |
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| Kit Capacity: This kit provides 900 μL of MojoSort™ Human CD63 Exosome Microbeads and 25 mL of MojoSort™ Exosome Buffer (10X). For use of this kit, a single test is defined as processing 0.5 mL of human biofluids (serum, heparin-treated plasma, cell culture supernatant or urine). Per test, we recommend separation using 20 μL of MojoSort™ Human CD63 Exosome Microbeads. This kit provides enough reagents to process approximately 20 mL of human biofluids.To obtain more MojoSort™ Exosome Buffer (10X), please contact Technical Services for more information at https://www.biolegend.com/en-us/contact.Starting sample notes: If you intend to select exosomes from < 0.5 mL of biofluids per test, please bring up the volume to 0.5 mL using 1X Exosome Buffer and adhere to indicated volumes for 0.5 mL tests according to the protocol. Please note that using less than the recommended volume of 0.5 mL of starting sample may result in reduced exosome yield. Levels of CD63 tetraspanins on the surface of exosomes may vary between source of biofluids (serum, heparin-treated plasma, cell culture supernatant or urine) and may also be donor dependent. Varying CD63 levels on human exosomes may also affect yield and require titration of kit components. We may see a faint non-specific band around 27 kD and 55 kD; this is due to the light chain being separated from the heavy chain (vice versa) and falling off from the antibody conjugated beads while lysing and preparing the bead bound exosomes for running in SDS gel in reduced form (because of denaturation with SDS in the sample buffer and reduction with beta-mercaptoethanol). This kit is validated for use with Heparin-treated plasma only. It is not suitable for other types of plasma.Antibody or cocktail dilution to use in column: N/A Dilution to use in column: N/A |
| Gene ID | NA |
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| UniProt | View information about CD63 on UniProt.org |
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| Regulatory Status | RUO |
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| Other Names | Human CD63 positive selection, Exosome positive selection, Exosome positive isolation |
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