Biolegend, 501817, Alexa Fluor® 647 anti-human IL-12/IL-23 p40 Antibody
25tests
Catalog Number: 501817
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | CHO-expressed, recombinant human IL-12 p70 heterod |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | Flow Cytometry3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization6: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813). Additional reported applications (for the relevant formats) include: Western blotting, ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4 of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry. Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended. |
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| Application References(PubMed link indicates BioLegend citation) | D'Andrea A, et al. 1992. J. Exp. Med. 176:1387. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041. Prussin C, et al. 1995. J. Immunol. Meth. 188:117. Leifeld L, et al. 2002. Hepatology 36:1001. Pekkari K, et al. 2001. Blood 97:3184. Bolovan-Fritts CA, et al. 2004. J. Virol. 78:13173. |
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| Product Citations | Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed Nishibayashi R, et al. 2015. PLoS One. 10:129806. PubMed |
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| RRID | AB_2124518 (BioLegend Cat. No. 501818) |
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| Structure | Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40) |
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| Bioactivity | IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human memory T cells, but not n |
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| Cell Sources | Dendritic cells, monocytes/macrophages, B cells, T cells |
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| Cell Targets | T cells, NK cells, PBL |
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| Receptors | IL-12Rß1 binds p40; dimeric with IL-12Rß2 binds p35 |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49. |
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| Regulation | Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23 |
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| Gene ID | 3593 |
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| UniProt | View information about IL-12/IL-23 p40 on UniProt.org |
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| Clone | C11.5 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF) |
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| Isotype | Mouse IgG1, κ |
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