Biolegend, 502516, Alexa Fluor® 647 anti-human IFN-γ Antibody
100tests
Catalog Number: 502516
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| Verified Reactivity | Human |
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| Reported Reactivity | Chimpanzee, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Partially purified, native human IFN-γ |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended. |
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| Application References(PubMed link indicates BioLegend citation) | Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut) Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut) Sester M, et al. 2002. J. Virol. 76:3748. (ICFC) Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC) Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA) Chen H, et al. 2005. J. Immunol. 175:591. (ICFC) Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC) Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC) |
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| Product Citations | Shamriz O, et al. 2023. Front Immunol. 13:1044933. PubMed Christian ML, et al. 2022. J Immunol. 208:1700. PubMed Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed Hoang TN, et al. 2021. Cell. 184:460. PubMed Groß R, et al. 2022. EBioMedicine. 75:103761. PubMed Sung BY, et al. 2022. J Clin Invest. 132:. PubMed Hoang TN, et al. 2020. bioRxiv. . PubMed Wang Z, et al. 2020. J Clin Invest. 130:3717. PubMed Oh DY, et al. 2020. Cell. 181:1612. PubMed Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Li W, et al. 2020. Immunity. 53(2):456-470. PubMed Lever M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6630 - E6638. PubMed Corrado M, et al. 2020. Cell Metab. 32:981. PubMed |
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| RRID | AB_493031 (BioLegend Cat. No. 502516) |
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| Structure | Cytokine; dimer; 20-25 kD (Mammalian) |
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| Bioactivity | Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC |
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| Cell Sources | CD8+ and CD4+ T cells, NK cells |
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| Cell Targets | T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts |
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| Receptors | IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1) |
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| Cell Type | Tregs |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151. |
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| Regulation | Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2 |
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| Gene ID | 3458 |
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| UniProt | View information about IFN-gamma on UniProt.org |
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| Clone | 4S.B3 |
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| Regulatory Status | RUO |
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| Other Names | Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma |
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| Isotype | Mouse IgG1, κ |
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