Biolegend, 503505, Biotin anti-mouse / rat IL-1β Antibody
50microg
Catalog Number: 503505
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| Verified Reactivity | Mouse, Rat |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | E. coli -expressed, recombinant mouse IL-1β |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ELISA - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. |
| Application Notes | ELISA or ELISPOT Capture3: The purified B122 antibody is useful as the capture antibody in a mouse IL-1ß sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated Poly5158 antibody (Cat. No. 515801) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture. Certain formats of clone B122 have been reported for in vitro neutralization of IL-1ß bioactivity1, in vivo cytokine depletion5, immunohistochemical staining of frozen sections6, Western blotting7, and the study of endotoxin shock8. |
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| Application References(PubMed link indicates BioLegend citation) | Hogquist K, et al. 1991. J. Immunol. 146:1534. Williams RO, et al. 2000. J. Immunol. 165:7240. (Neut) Brewer J, et al. 1998. J. Immunol. 161:4000. (ELISA and ELISPOT Capture) Rogers HW, et al. 1992. Proc. Natl. Acad.Sci. USA 89:1011. Cantwell AM, et al. 2010. BMC Immunol. 11:29. (Depletion) Dube PH, et al. 2001. Proc. Natl. Acad. Sci. USA 98:10880. (IHC) Babelova A, et al. 2009. J. Biol. Chem. 284:24035. (WB) Lu JY, et al. 2006. Genes Dev. 20:3174. (Neut) PubMed |
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| Product Citations | Bulek K, et al. 2020. J Clin Invest. 130:4218. PubMed Allen KD, et al. 2020. Connect Tissue Res. 61:82. PubMed |
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| RRID | AB_2295906 (BioLegend Cat. No. 503505) |
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| Structure | Cytokine; 17.5 kD (Mammalian) |
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| Bioactivity | Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages |
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| Cell Sources | Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, |
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| Cell Targets | B cells, T cells, monocytes |
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| Receptors | Type I IL-1R (CDw121a), Type II IL-1R (CDw121b) |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095. |
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| Regulation | Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin |
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| Gene ID | 16176 |
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| UniProt | View information about IL-1beta on UniProt.org |
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| Clone | B122 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF) |
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| Isotype | Armenian Hamster IgG |
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