Biolegend, 505302, Biotin anti-mouse IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody
500microg
Catalog Number: 505302
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | CHO expressed, recombinant mouse IL-12 p70 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ELISA Detection, ELISPOT Detection, ICFC |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. For use as an ELISPOT detection antibody, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| ELISA Detection1,2,6-9 or ELISPOT Detection: The biotinylated C17.8 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified C15.6 antibody (Cat. No. 505202/505208) as capture antibody and recombinant mouse IL-12/IL-23 p40 (Cat. No. 576909) or recombinant mouse IL-12 p70 (Cat. No. 577009) as the standard. Neutralization1,2,4,5,6,10: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-12 bioactivity in vivo and in vitro (Cat. No. 505304). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505308) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). Additional reported applications (for relevant formats of this clone) include: Western blotting3, immunoprecipitation3, and intracellular immunofluorescent staining and flow cytometric analysis. Note: For testing mouse IL-12 p40 (monomer, dimer, heterodimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431601 to 431606) are specially developed and recommended. |
| Application References(PubMed link indicates BioLegend citation) | Kitagaki K, et al. 2002. Clin. Diagn. Lab Immunol. 9:1260. Reichmann G, et al. 1999. J. Immunol. 163:3354. Wysocka M, et al. 1995. Eur. J. Immunol. 25:672. Neucath M, et al. 1995. J. Exp. Med. 182:1281. Cousens LP, et al. 1999 J. Exp. Med. 189:1315. Gately M. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.16. O'Connell PJ, et al. 2006. Blood 107:1010. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. Xu G, et al. 2007. J. Immunol. 179:5358. PubMed Sugimoto K, et al. 2007. Gastroenterology 133:124. PubMed |
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| Product Citations | Yan H, et al. 2022. Biomater Adv. 139:213009. PubMed Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed Liang D, et al. 2018. Front Immunol. 8:1801. PubMed Bourquin C, et al. 2009. J Immunol. 183:6078. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed Yan H, et al. 2019. Sci Rep. 9:10447. PubMed Luo X, et al. 2021. Neuron. 109:2691. PubMed |
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| RRID | AB_315373 (BioLegend Cat. No. 505301) AB_315374 (BioLegend Cat. No. 505302) |
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| Structure | Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40) |
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| Bioactivity | IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me |
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| Cell Sources | Dendritic cells, monocytes/macrophages, B cells, T cells |
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| Cell Targets | T cells, NK cells |
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| Receptors | IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35 |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux V. 1992. Research Immunol. 143:385. 3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355. 4. Trinchieri G, et al. 1993 Immunol. Today. 14:335. 5. Oppmann B, et al. 2000 Immunity. 13:715. 6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910. 7. Parham C, et al. 2002 J. Immunol. 168:5699. 8. Belladonna ML, et al. 2002 J. Immunol. 168:5448. 9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49. |
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| Regulation | Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23 |
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| Gene ID | 16160 |
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| UniProt | View information about IL-12/IL-23 p40 on UniProt.org |
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| Clone | C17.8 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF) |
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| Isotype | Rat IgG2a, κ |
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