Biolegend, 505816, Alexa Fluor® 647 anti-mouse IFN-γ Antibody
25microg
Catalog Number: 505816
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Rat |
| Immunogen | E. coli-expressed, recombinant mouse IFN-γ |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
| Excitation Laser |
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| Red Laser (633 nm) |
| Application Notes | ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812). Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations. Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg). Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry. Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended. |
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| Application References(PubMed link indicates BioLegend citation) | Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut) Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut) Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut) Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA) Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT) Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC) Ferrick D, et al. 1995. Nature 373:255. (FC) Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut) DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut) Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed Montfort M, et al.2004. J. Immunol. 173:4084. PubMed Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC) Mottram PL, et al. 1998. J Immunol. 161:602. (IHC) |
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| Product Citations | Calabrese DR, et al. 2020. J Clin Invest. . PubMed Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed Zhao X, et al. 2022. Cell Mol Immunol. 19:540. PubMed Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed Porsche CE, et al. 2021. JCI Insight. 6:. PubMed Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed Shrivastava T, et al. 2021. Front Immunol. 12:641447. PubMed Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed De Pascalis R, et al. 2020. PLoS One. 15:e0237034. PubMed Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed Chang Y, et al. 2015. JPEN J Parenter Enteral Nutr. . PubMed Montfort M, et al. 2004. J Immunol. 173:4084. PubMed Majedi FS, et al. 2020. Biomaterials. 252:120058. PubMed Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed Lee J, et al. 2007. Nat Immunol. 8:181. PubMed Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed Chakraborty P, et al. 2020. Cell Reports. 28(7):1879-1893.e7.. PubMed Sasai M, et al. 2021. J Exp Med. 218:. PubMed Long L, et al. 2021. Nature. 600:308. PubMed Ying B, et al. 2022. Sci Transl Med. 14:eabm3302. PubMed |
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| RRID | AB_493315 (BioLegend Cat. No. 505816) AB_493314 (BioLegend Cat. No. 505814) |
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| Structure | Cytokine; dimer; 40-80 kD (Mammalian) |
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| Bioactivity | Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs |
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| Cell Sources | CD8+ and CD4+ T cells, NK cells |
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| Cell Targets | T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts |
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| Receptors | IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1) |
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| Cell Type | Tregs |
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| Biology Area | Cell Biology, Immunology, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321. 3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571. 4. Gray P, et al. 1987. Lymphokines 13:151. |
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| Regulation | Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone |
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| Gene ID | 15978 |
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| UniProt | View information about IFN-gamma on UniProt.org |
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| Clone | XMG1.2 |
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| Regulatory Status | RUO |
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| Other Names | Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF) |
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| Isotype | Rat IgG1, κ |
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