Biolegend, 505909, APC anti-mouse/rat/human MCP-1 Antibody
25microg
Catalog Number: 505909
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| Verified Reactivity | Mouse, Rat, Human |
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| Reported Reactivity | Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Hamster |
| Immunogen | CHO-expressed, recombinant mouse MCP-1 |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. |
| Concentration | 0.2 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Excitation Laser | Red Laser (633 nm) |
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| Application Notes |
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| ELISA or ELISPOT Capture1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization. Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7. |
| Application References(PubMed link indicates BioLegend citation) | Luo, Y., et al. 1994. J. Immunol. 153:3708. Zhang, Y., et al. 2002. J. Immunol. 168:3088. Luo, Y., et al. 1999 J. Immunol. 163:3985. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862. Hancock, W. W., et al. 1997 Transplantation 64:696. Yu, R., et al. 2006 Obesity 14:1353. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed |
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| Product Citations | Campisi L, et al. 2022. Nature. 606:945. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed |
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| RRID | AB_2566053 (BioLegend Cat. No. 505909) AB_2566054 (BioLegend Cat. No. 505910) |
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| Structure | CC chemokine; dimer; 13.8 kD (Mammalian) |
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| Bioactivity | Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells |
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| Cell Sources | Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells |
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| Cell Targets | Monocytes, basophils, T cells, NK cells, immature dendritic cells |
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| Receptors | CCR2; CCR5; CCR10; D6; possibly L-CCR |
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| Biology Area | Cell Biology, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271. 3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang, Y., et al. 1992. J. Immunol. 148:2423. |
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| Regulation | Upregulated by IL-1, TNF-α, PDGF, TGF-β |
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| Gene ID | 20296247706347 |
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| UniProt | View information about CCL2 on UniProt.org |
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| Clone | 2H5 |
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| Regulatory Status | RUO |
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| Other Names | Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2 |
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| Isotype | Hamster IgG |
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