Biolegend, 507214, Alexa Fluor® 647 anti-human Granzyme A Antibody
100tests
Catalog Number: 507214
Send an RFQ.
Category
Loading…
| Verified Reactivity | Human |
|---|---|
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified human Granzyme A |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
| Excitation Laser | Red Laser (633 nm) |
|---|
| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2. |
|---|
| Application References(PubMed link indicates BioLegend citation) | Trimble L, et al. 1998. Blood 91:585. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. Raqib R, et al. 2002. Infect. Immun. 70:3199. Chen H, et al. 2005. J. Immunol. 175:591. |
|---|
| Product Citations | Ren Y, et al. 2018. J Virol. :92. PubMed Kiniry B, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.100. PubMed Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed Chen H, et al. 2021. iScience. 24:103387. PubMed Shen Z, et al. 2022. Immun Ageing. 19:55. PubMed |
|---|
| RRID | AB_2114395 (BioLegend Cat. No. 507214) |
|---|
| Structure | Serine protease; disulfide-linked homodimer; 28 kD (Mammalian) |
|---|
| Ligand/Receptor | Assisted by perforin |
|---|
| Bioactivity | Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking |
|---|
| Cell Sources | Cytotoxic T cells, NK cells |
|---|
| Cell Targets | Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1 |
|---|
| Receptors | Assisted by perforin |
|---|
| Cell Type | Tregs |
|---|
| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
|---|
| Molecular Family | Enzymes and Regulators, Proteases |
|---|
| Antigen References | 1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679. |
|---|
| Gene ID | 3001 |
|---|
| UniProt | View information about Granzyme A on UniProt.org |
|---|
| Clone | CB9 |
|---|
| Regulatory Status | RUO |
|---|
| Other Names | Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3) |
|---|
| Isotype | Mouse IgG1, κ |
|---|
