Biolegend, 507218, Alexa Fluor® 594 anti-human Granzyme A Antibody
100microg
Catalog Number: 507218
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| Verified Reactivity | Human |
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| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Purified human Granzyme A |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. |
| Concentration | 0.5 mg/mL |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested ICC - Verified |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
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| Application Notes | Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2. |
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| Application References(PubMed link indicates BioLegend citation) | Trimble L, et al. 1998. Blood 91:585. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285. Raqib R, et al. 2002. Infect. Immun. 70:3199. Chen H, et al. 2005. J. Immunol. 175:591. |
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| RRID | AB_2563964 (BioLegend Cat. No. 507218) |
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| Structure | Serine protease; disulfide-linked homodimer; 28 kD (Mammalian) |
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| Bioactivity | Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking |
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| Cell Sources | Cytotoxic T cells, NK cells |
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| Cell Targets | Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1 |
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| Receptors | Assisted by perforin |
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| Cell Type | Tregs |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroscience |
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| Molecular Family | Enzymes and Regulators, Proteases |
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| Antigen References | 1. Brune J, et al. 1986. Nature 322:268. 2. Fan Z, et al. 2003. Nature Immunol. 4:145. 3. Fan Z, et al. 2003. Cell 112:659. 4. Masson D, et al. 1987. Cell 49:679. |
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| Gene ID | 3001 |
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| UniProt | View information about Granzyme A on UniProt.org |
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| Clone | CB9 |
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| Regulatory Status | RUO |
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| Other Names | Granzyme 1, Hanukah factor serine protease (HFSP), Cytotoxic T-lymphocyte-associated serine esterase 3 (CTLA-3) |
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| Isotype | Mouse IgG1, κ |
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