Biolegend, 508207, Alexa Fluor® 647 anti-human IL-1β Antibody
25tests
Catalog Number: 508207
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| Verified Reactivity | Human |
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| Reported Reactivity | Baboon |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | Recombinant human IL-1 beta |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | ICFC - Quality tested |
| Recommended Usage | Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses |
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Excitation Laser | Red Laser (633 nm) |
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| Application Notes | ELISA and ELISPOT Capture: The purified JK1B-1 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated JK1B-2 antibody (Cat. No. 508301) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.Flow Cytometry: The fluorochrome-labeled JK1B-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-1β-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. |
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| Application References(PubMed link indicates BioLegend citation) | Lu Y, et al. 2015. PNAS. 112:607. PubMed |
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| Product Citations | Sharma SK, et al. 2013. Vaccine. 3090:31. PubMed Zheng W, et al. 2022. Front Immunol. 13:926087. PubMed Fitzgerald KC, et al. 2021. Cell Rep Med. 2:100424. PubMed Fell L, et al. 2014. Nephrol Dial Transplant. 29:809. PubMed Loiarro M, et al. 2013. J Biol Chem. 288:30210. PubMed |
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| RRID | AB_604133 (BioLegend Cat. No. 508207) AB_604135 (BioLegend Cat. No. 508208) |
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| Structure | Cytokine; 17.3 kD (Mammalian) |
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| Bioactivity | Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia |
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| Cell Sources | Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes, |
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| Cell Targets | B cells, T cells, monocytes |
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| Receptors | Type I IL-1R (CDw121a), Type II IL-1R (CDw121b) |
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| Biology Area | Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience |
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| Molecular Family | Cytokines/Chemokines |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York. 3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241. 4. Dinarello C. 1996. Blood 87:2095. |
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| Regulation | Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin |
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| Gene ID | 3553 |
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| UniProt | View information about IL-1beta on UniProt.org |
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| Clone | JK1B-1 |
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| Regulatory Status | RUO |
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| Other Names | Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF) |
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| Isotype | Mouse IgG1, κ |
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