Biolegend, 509801, Biotin anti-mouse/human NGF Antibody
50microg
Catalog Number: 509801
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| Verified Reactivity | Human |
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| Reported Reactivity | Mouse, Cow, Viper |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Immunogen | recombinant human NGF (c-terminus) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. |
| Application | ELISA Detection |
| Recommended Usage | Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1 - 3 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. Additionally, BioLegend recommends the usage of Tris-Buffered Saline (TBS), pH = 7.2 for all washes and incubations, except substrate buffer. Do not use PBS as results may vary dramatically. |
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| Application Notes |
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| ELISA Detection (mouse): The biotinylated JKhNGF-2 antibody is useful as the detecting antibody in a sandwich ELISA assay, when used in conjunction with the purified JKmNGF-1 antibody (Cat. No. 509702) as the capture antibody for detecting mouse NGF. ELISA Detection (human): The biotinylated JKhNGF-2 antibody is useful as the detecting antibody in a sandwich ELISA assay, when used in conjunction with the purified JKhNGF-1 antibody (Cat. No. 509602) as the capture antibody for detecting human NGF. |
| RRID | AB_2152401 (BioLegend Cat. No. 509801) AB_2152400 (BioLegend Cat. No. 509802) |
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| Structure | Kallikrein family of serine proteases; dimer; 13.5 kD (Mammalian) |
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| Bioactivity | Enhances survival, growth, neurotransmitter biosynthesis of sympathetic, sensory neurons; neurotrophic factor; cutaneous innervation; growth, differentiation, survival B lymphocytes; possible role in allergy, tissue repair |
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| Cell Sources | Hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells, eosinophils |
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| Cell Targets | Neurons, B cells, mast cells, eosinophils |
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| Receptors | Low-affinity NGF receptor (LNGFR, p75 LNGFR, fast NGF receptor); high-affinity NGF receptor (trkA) |
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| Molecular Family | Cytokines/Chemokines, Growth Factors |
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| Antigen References | 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Levi A, et al. 1991. Annu. Rev. Pharmacol. Toxicol. 31:205. 3. Varon S, et al. 1991. Adv. Exp. Med. Biol. 296:267. 4. Micera A, et al. 2003. Cytokine Growth Factor Rev. 14:369. |
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| Regulation | Upregulated by glutamate, vitamin D3, IL-6, FGF-basic, astrocyte-specific IL-1, TNF-α, PDGF, TGF-β; downregulated by GABAergic neuronal activity, glucocorticoids, Schwann cell-specific TGF-β |
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| Gene ID | 180494803 |
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| UniProt | View information about NGF on UniProt.org |
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| Clone | JKhNGF-2 |
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| Regulatory Status | RUO |
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| Other Names | Nerve growth factor, NGF, β-NGF |
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| Isotype | Mouse IgG1 |
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